object_id	card_schema	card_version	resource_release	evidence_freeze	interface_build	gene	card_type	current_action_code	current_action	lifecycle	decision_blocking_gap	highest_missing_evidence_layer	secondary_gaps	frozen_evidence_snapshot	unresolved_question	biological_context	perturbation_modality	comparator_and_controls	primary_endpoint	secondary_endpoints	primary_estimand	state_transition_design	perturbation_direction_rationale	minimally_important_effect	negligible_effect_margin	suggested_sample_size_range	randomisation_unit	blocking_factors	statistical_model	multiple_testing_family	missing_data_rule	assay_qc_thresholds	guide_concordance_rule	donor_replication_rule	estimated_time_band	estimated_cost_band	preregistration_status	inferential_unit	evidence_import_requirements	planning_fields_not_applicable	stop_rules	escalation_rule	evidence_transition	data_release_plan	boundary	stable_url	exports
SC:v1.0.0-database-resource:GFOD2:1.5-DRAFT	nmd-vcell-study-card/1.5	1.5	v1.0.0-database-resource	2026-07-25	EA-20260729-18	GFOD2	evidence_import_audit	hold_pending_external_audit	Provisional hold · external report not yet audited	{"state":"DRAFT","revision":1,"immutable":false,"registered_at":null,"supersedes":null,"immutable_after_registration":true}	{"code":"GAP-09","label":"External quantitative evidence has not been imported and audited"}	L4_dmd_functional_validation	GAP-02 independent muscle-context perturbation | GAP-03 DMD-relevant functional validation | GAP-07 independent replication	{"snapshot_schema":"nmd-vcell-study-card-frozen-evidence-snapshot/1.0","source_record":"gene/GFOD2","highest_supported_level":"L2_observed_same_context_perturbation","highest_assessed_level":"L2_observed_same_context_perturbation","observed_hepg2_perturbation":{"assessment_status":"assessed","support_status":"supported","observed_cells":127},"external_context_screen":{"assessment_status":"not_assessed","support_status":null,"hit":null,"effect_size":null,"fdr":null,"interpretation":null},"dmd_prior":{"integrated_prior_direction":"up","source_direction_state":"conflicted","source_agreement_proportion":0.5,"agreeing_sources":2,"assessed_sources":4,"coverage_state":"4_of_4_sources","uncertainty_state":"direction_conflict_retained"},"context_metrics":{"skeletal_muscle_median_tpm":2.94732,"depmap_median_gene_effect":-0.0768458591182602,"moderate_dependency_flag":false},"visual_boundary":"Descriptive frozen evidence snapshot only; no score, rank, cluster, prediction claim or intervention recommendation is generated."}	Does the author-reported external evidence for GFOD2 meet provenance, context, independence and negative-evidence criteria?	External-evidence audit only; no new biological escalation until the quantitative import is complete.	None. This card imports and audits an existing evidence object.	Dataset accession and source owner. | Raw/processed availability. | Target mapping and assay context. | Primary endpoint, effect size and uncertainty. | Independent reproduction and import acceptance criteria.	Completeness, reproducibility and claim-ceiling classification of the imported quantitative result.	Provenance completeness. | Context match. | Negligible-effect interpretation. | Independent reproduction status.	Imported effect estimate with its uncertainty, denominator and exact contrast; no reconstructed estimate is accepted without provenance.	{"scientific_object":"perturbation × cell state × disease context × time × phenotype","mechanism_hypothesis":"Required before registration: state the proposed early molecular mediator, the expected cell-state transition and the downstream functional consequence.","cell_context_fields":{"disease_background":"Required before registration: healthy, DMD or isogenic corrected.","myogenic_state":"Required before registration: proliferating myoblast, early differentiation, fusion or maturing myotube.","donor_or_isogenic_pair":"Required before registration; preserve donor-specific estimates."},"timepoint_plan":[{"window":"6–12 h","role":"early molecular or signalling response","status":"planning_default_requires_assay_calibration"},{"window":"24–48 h","role":"regulatory program and cell-state transition","status":"planning_default_requires_assay_calibration"},{"window":"4–7 d","role":"differentiation and functional phenotype","status":"planning_default_requires_assay_calibration"}],"endpoint_domains":{"target_engagement":["mRNA","protein where validated","perturbation efficiency"],"functional":["fusion","morphology","membrane integrity","calcium","contraction"],"safety":["viability","proliferation","differentiation blockade","global stress"],"replication":["reagent","donor or isogenic pair","future batch"]},"cell_cell_consequence":{"current_status":"not_assessed","future_levels":["conditioned medium","two-cell co-culture","three-dimensional muscle model","spatial perturbation model"]},"response_archetype":{"current_status":"not_assessed","allowed_values":["robust_responder","dmd_specific_responder","donor_variable","state_specific","toxic_responder","null_with_equivalence_margin","discordant","qc_failure","inconclusive"]}}	{"selected_hypothesis":"not_applicable","allowed_hypotheses":["not_applicable"],"required_justification":"No new perturbation direction is nominated by an evidence-import audit."}					Source study. | Assay batch. | Biological context. | Analysis version.	Reproduce the source model exactly when code and inputs permit; otherwise classify as not reproducible.	Preserve the source family and correction; do not reinterpret an isolated P value.	Define exclusions before unblinding; report all missing units and reasons; do not single-impute primary outcomes without a prespecified sensitivity analysis.	Accession resolvable. | Raw or sufficient processed data available. | Target mapping exact. | Effect, interval and denominator present. | Code/provenance sufficient for reproduction.	Not applicable unless the imported study reports multiple reagents; if so, preserve reagent-specific results.	Record whether the source result replicates across donors/samples; absence is a limitation, not a negative result.	2–10 working days after complete source delivery; planning estimate only.	Institution- and assay-dependent; obtain a local itemised quote before registration.	draft_requires_source_accession_denominator_contrast_and_reproducibility_audit	Required import field: preserve the source study’s declared inferential unit exactly.	Source accession and owner. | Raw and processed data availability. | Exact denominator and exclusion flow. | Effect estimate, interval and primary contrast. | Original allocation and inferential unit. | Preregistration status. | Code and environment reproducibility. | Reagent and donor replication. | Negative-result definition. | Selective-reporting assessment.	minimally_important_effect | negligible_effect_margin | suggested_sample_size_range | randomisation_unit	Stop or classify as infeasible if a required numeric QC threshold fails. | Do not interpret a nonsignificant result as no material effect without a negligible-effect interval. | Do not change canonical candidate status automatically; require governed review.	Retain, release or formally change the hold only after provenance, independence and negative-evidence gates are audited.	Author report pending import → audited evidence object → governed status decision.	Release the frozen card, protocol identifiers, analysis code, complete denominators and results irrespective of direction; never overwrite the registered card.	This Study Card is an evidence-gated design scaffold, not a protocol, power calculation, safety claim, prediction or therapeutic recommendation.	study-card/GFOD2	{"json":"api/v1.1/study-cards/GFOD2.json","yaml":"downloads/study-cards/GFOD2.yaml","tsv":"downloads/study-cards/GFOD2.tsv"}