# NMD-VCell disease-prediction platform gate

This release is a prediction-infrastructure candidate, not a validated disease
prediction platform.

## Current state

- Resource release: `v1.0.0-database-resource`
- Model release: `ridge-safe-v2.3`
- Current level: `P0_prediction_infrastructure_only`
- Recommended next level: `P1_research_use_dmd_perturbation_response_prediction`
- Clinical level: `P2_clinical_prediction_locked`

## What is allowed now

- static prediction-registration schema and empty registry are available
- same-HepG2 perturbation-response benchmark is auditable
- future prospective outcomes can be linked without overwriting predictions
- evidence gaps and abstention requirements are explicit

## What remains locked

- validated DMD disease predictor
- therapeutic response predictor
- clinical decision-support platform
- treatment-prioritization engine

## Minimum P1 unlock package

- frozen DMD/dystrophin-deficient human myogenic or neuromuscular perturbation dataset
- train/validation/external-test partition declared before model fitting
- donor, isogenic-pair or perturbation unit of analysis declared
- negative, positive and rescue/control definitions predeclared
- primary functional endpoint and transcriptomic secondary endpoints frozen
- baseline models, calibration, uncertainty intervals and abstention policy benchmarked
- out-of-distribution detection and failure-mode taxonomy frozen
- prospective prediction cards timestamped before outcome access
- outcome board populated without relabelling failed or abstained predictions

## Boundary

No P1 or P2 prediction claim can be made until independent disease-relevant perturbation data, prospective registration, external test results, calibration, uncertainty, OOD and abstention analyses are frozen.